Journal
/
Ingeniería
/
Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche
JoVE Revista
Ingeniería
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Ingeniería
Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
Please note that all translations are automatically generated. Click here for the English version.

Arricchire ed espandere cellule T antigene-specifiche rara con nanoparticelle magnetiche

11,276 Views

•

09:28 min

•

November 17, 2018

DOI:

10.3791/58640-v

DOI:
10.3791/58640-v

•

09:28 min
November 17, 2018

•

6 Views
,
1Department of Biomedical Engineering, School of Medicine,Johns Hopkins University, 2Institute for Cell Engineering, School of Medicine,Johns Hopkins University, 3Institute for Nanobiotechnology,Johns Hopkins University, 4Department of Pathology, School of Medicine,Johns Hopkins University

Summary

Automatically generated

Cellule T antigene-specifiche sono difficili da caratterizzare o utilizzare in terapie grazie alla loro frequenza estremamente bassa. Qui, forniamo un protocollo per sviluppare una particella magnetica che si può associare a cellule T antigene-specifiche per arricchire queste cellule e quindi di espanderli diversi moltiplicati per la caratterizzazione e la terapia.

Explore More Videos

Magnetic NanoparticlesAntigen-specific T CellsEnrichmentExpansionNeoepitopesArtificial Antigen-presenting CellsImmunotherapyCellular TherapyResuspension BufferStimulatory SignalsQuenching Buffer

Read Article

Cite this Article

Hickey, J. W., Schneck, J. P. Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles. J. Vis. Exp. (141), e58640, doi:10.3791/58640 (2018).

Download .ris file

Copy

Share Video

.

Copy