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如何定量光活化荧光蛋白在大容量和活细胞中的分数

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells

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如何定量光活化荧光蛋白在大容量和活细胞中的分数

6,480 Views

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11:03 min

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January 07, 2019

DOI:

10.3791/58588-v

DOI:

10.3791/58588-v

•

11:03 min

January 07, 2019

•

1 Views

Vanessa Chen¹, Malte Renz¹

¹Gynecologic Oncology Division,Stanford University School of Medicine

Summary

在这里, 我们提出了一个协议, 涉及基因耦合光谱不同的光可激活和荧光蛋白。这些荧光蛋白嵌合体允许定量的 pa-fp 分数是光活化的荧光,即光活化效率。该协议揭示了不同的光活化模式产生不同的光活化效率。

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