/
/
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
6,497 Views
•
11:03 min
•
January 07, 2019
DOI:
Summary
Automatically generated
Here, we present a protocol that involves genetically coupled spectrally distinct photoactivatable and fluorescent proteins. These fluorescent protein chimeras permit quantification of the PA-FP fraction that is photoactivated to be fluorescent, i.e., the photoactivation efficiency. The protocol reveals that different modes of photoactivation yield different photoactivation efficiencies.
Read Article
Cite this Article
Chen, V., Renz, M. How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells. J. Vis. Exp. (143), e58588, doi:10.3791/58588 (2019).
Copy