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Kvantitative Polymerase Chain Reaction-baserede analyser af Murine tarmens mikrobiota efter peroral antibiotisk behandling
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment
DOI:

08:33 min

November 17, 2018

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Capítulos

  • 00:04Título
  • 00:39Administration of Antibiotics
  • 02:29Collection of Fecal Samples from Stool, Ileum Content and Ileum Wall
  • 05:16Quantification of Intestinal Microbiota by qPCR
  • 06:33Results: Quantification of Intestinal Microbiota by qPCR after Oral Antibiotic Treatment
  • 07:51Conclusion

Summary

Traducción Automática

Her leverer vi detaljerede protokoller til oral administration af antibiotika til mus, samling af fækal prøver, DNA-ekstraktion og kvantificering af fækal bakterier af qPCR.

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