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Inmunología y contagio

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Co-immunoprecipitation analysen for å studere funksjonell samhandling mellom reseptorer og enzymer

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation analysen for å studere funksjonell samhandling mellom reseptorer og enzymer

14,536 Views

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09:40 min

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September 28, 2018

DOI:

10.3791/58433-v

DOI:

10.3791/58433-v

•

09:40 min

September 28, 2018

•

3 Views

Michael Peled, Marianne Strazza, Adam Mor

¹Pulmonary Department,The Chaim Sheba Medical Center, ²Columbia Center for Translational Immunology, Department of Medicine,Columbia University Medical Center

Tags

Co-immunoprecipitation Receptor-enzyme Interaction Cell Signaling Protein Domains Enzymatic Activation DMEM 293-T Cells Transfection GFP Expression Pervanadate Phosphorylation PD-1 Lysis Buffer Protease Inhibitors Sodium Orthovanadate Immunoprecipitation

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