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포유류 세포에서 효율 Resealing 원형질 막의 높은 처리량 측정
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
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포유류 세포에서 효율 Resealing 원형질 막의 높은 처리량 측정

7,594 Views

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10:07 min

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January 07, 2019

DOI:

10.3791/58351-v

DOI:
10.3791/58351-v

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10:07 min
January 07, 2019

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5 Views
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1Department of Microbial Infection and Immunity,The Ohio State University, 2Department of Microbiology,The Ohio State University, 3Infectious Diseases Institute,The Ohio State University, 4Division of Biostatistics, College of Public Health,The Ohio State University

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Plasma Membrane ResealingHigh-throughputHeLa Cells96-well PlateFluorescencePropidium IodideKinetic AssayPlate ReaderOptical ConfigurationRead ModeRead Type

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Lam, J. G., Song, C., Seveau, S. High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells. J. Vis. Exp. (143), e58351, doi:10.3791/58351 (2019).

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