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氧电极对 DesB 酶活性和抑制作用的厌氧蛋白纯化及动力学分析
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October 03, 2018
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在这里, 我们提出了一个协议的厌氧蛋白纯化, 厌氧蛋白浓度, 并随后的动力学表征使用氧电极系统。该方法用酶 DesB, 一种在厌氧环境中纯化和贮存时更稳定、更活跃的酶酶来说明。
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AnaerobicProtein PurificationKinetic AnalysisOxygen ElectrodeDesB DioxygenaseEnzyme ActivityInhibitionInorganic BiochemistryOxygen-sensitive MetalsProtein ExpressionCell PelletAmylose ColumnLysozymeHigh-pressure HomogenizationNitrogen GasGlove BoxAmylose Column BufferElution BufferFerrous Ammonium SulfateDithiothreitol
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Uchendu, S. N., Rafalowski, A., Cohn, E. F., Davoren, L. W., Taylor, E. A. Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition. J. Vis. Exp. (140), e58307, doi:10.3791/58307 (2018).
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