/
/
Antigenic Liposomes for Generation of Disease-specific Antibodies
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Antigenic Liposomes for Generation of Disease-specific Antibodies
12,173 Views
•
10:31 min
•
October 25, 2018
DOI:
1Janssen R&D, 2Department of Microbiology and Immunology,University of North Carolina, 3UNC Food Allergy Initiative,University of North Carolina, 4Department of Pediatrics,University of North Carolina, 5Department of Chemistry,University of Alberta, 6Department of Molecular Medicine,Scripps Research Institute, 7Department of Medical Microbiology and Immunology,University of Alberta
Summary
Automatically generated
Described is the preparation of antigenic liposomal nanoparticles and their use in stimulating B-cell activation in vitro and in vivo. Consistent and robust antibody responses led to the development of a new peanut allergy model. The protocol for generating antigenic liposomes can be extended to different antigens and immunization models.
Read Article
Cite this Article
Bednar, K. J., Hardy, L., Smeekens, J., Raghuwanshi, D., Duan, S., Kulis, M. D., Macauley, M. S. Antigenic Liposomes for Generation of Disease-specific Antibodies. J. Vis. Exp. (140), e58285, doi:10.3791/58285 (2018).
Copy