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利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究

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Inmunología y contagio

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JoVE Revista Inmunología y contagio

Generation of Knock-out Primary and Expanded Human NK Cells Using Cas9 Ribonucleoproteins

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利用 Cas9 Ribonucleoproteins 生成人 NK 细胞的研究

DOI:

10.3791/58237-v

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07:20 min

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June 14, 2018

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Meisam Naeimi Kararoudi, Hamid Dolatshad, Prashant Trikha, Syed-Rehan A. Hussain, Ezgi Elmas, Jennifer A. Foltz, Jena E. Moseman, Aarohi Thakkar, Robin J. Nakkula, Margaret Lamb, Nitin Chakravarti, K. John McLaughlin, Dean A. Lee

¹Center for Childhood Cancer and Blood Disease,Nationwide Children’s Hospital, ²Nuffield Division of Clinical Laboratory Sciences, Radcliffe Department of Medicine,University of Oxford, ³Center for Clinical and Translational Research,Nationwide Children’s Hospital Research Institute

Capítulos

00:04Título
00:44Human Primary and Expanded Natural Killer (NK) Cell Transduction
04:48Results: Representative Electroporation Efficiency
06:13Conclusion

Summary

Traducción Automática

在这里, 我们提出了一个协议, 基因改造主要或扩大人类自然杀手 (NK) 细胞使用Cas9 Ribonucleoproteins (Cas9/RNPs)。通过使用该协议, 我们生成的人 NK 细胞缺乏转化生长 factor–b 受体 2 (TGFBR2) 和嘌呤 phosphoribosyltransferase 1 (HPRT1)。

Tags

Cas9 Ribonucleoproteins NK Cells Gene Knockout Primary NK Cells Expanded NK Cells CRISPR RNA TracrRNA Electroporation Transduction Immunotherapy Solid Tumors Brain Tumors TGF-beta

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