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烟草 Benthamiana叶在制备鳞片上的瞬态表达
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August 16, 2018
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烟草 benthamiana叶片中生物合成酶的瞬态异源表达可以将 23-oxidosqualene 的内源供给转移到新的高附加值三萜产品的生产中。这里描述了一个详细的协议, 快速 (5 天) 准备规模生产萜和类似物利用这个强大的植物基础平台。
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Transient ExpressionNicotiana BenthamianaTriterpene ProductionPreparative ScaleAgrobacterium TumefaciensCo-infiltrationVacuum InfiltrationMMA BufferOD 600Preparative-scale AccessStructural CharacterizationMedicinally Relevant Biological ActivitySelective LB AgarSelective LB MediaCentrifugationResuspensionInfiltration Suspension
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Stephenson, M. J., Reed, J., Brouwer, B., Osbourn, A. Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale. J. Vis. Exp. (138), e58169, doi:10.3791/58169 (2018).
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