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단일 분자 힘 분광학에 대 한 단백질의 화학식 동원 정지

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Please note that all translations are automatically generated. Click here for the English version.

단일 분자 힘 분광학에 대 한 단백질의 화학식 동원 정지

10,576 Views

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11:13 min

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August 20, 2018

DOI:

10.3791/58167-v

DOI:

10.3791/58167-v

•

11:13 min

August 20, 2018

•

2 Views

Tanja D. Becke^2,3, Stefan Ness, Stefanie Sudhop³, Hermann E. Gaub, Markus Hilleringmann, Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Tags

Covalent Immobilization Protein Single Molecule Force Spectroscopy Atomic Force Microscope Cantilever Probe Biomolecule Immobilization Silanization EDC NHS Phosphate-buffered Saline

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