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Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

DOI:
10.3791/58167-v

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11:13 min

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August 20, 2018

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, , , , , ,
1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Capítulos

  • 00:04Título
  • 00:39Immobilization of Proteins via Functional Silane Coupling Agents
  • 04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
  • 08:38Results: Single Force Spectroscopy
  • 10:07Conclusion

Summary

Traducción Automática

Traducción Automática

This protocol describes the covalent immobilization of proteins with a heterobifunctional silane coupling agent to silicon-oxide surfaces designed for the atomic force microscopy based single molecule force spectroscopy which is exemplified by the interaction of RrgA (pilus-1 tip adhesin of S. pneumoniae) with fibronectin.

Tags

Covalent ImmobilizationProteinSingle Molecule Force SpectroscopyAtomic Force MicroscopeCantilever ProbeBiomolecule ImmobilizationSilanizationEDCNHSPhosphate-buffered Saline

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