Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
9,989 Views
•
09:06 min
•
October 05, 2018
DOI:
Summary
Automatically generated
The identification of physical interactions between genes and regulatory elements is challenging but has been facilitated by chromosome conformation capture methods. This modification to the 4C-seq protocol mitigates PCR bias by minimizing over-amplification of PCR templates and maximizes the mappability of reads by incorporating an addition restriction enzyme digest step.
Read Article
Cite this Article
Brettmann, E. A., Oh, I. Y., de Guzman Strong, C. High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq). J. Vis. Exp. (140), e58030, doi:10.3791/58030 (2018).
Copy