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Benchtop Metal benzeşme kromatografi, sulandırma immobilize ve bir Polyhistidine tahlil Metalloenzyme Lisans Laboratuvarı etiketli.
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Bioquímica
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JoVE Revista Bioquímica
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
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Benchtop Metal benzeşme kromatografi, sulandırma immobilize ve bir Polyhistidine tahlil Metalloenzyme Lisans Laboratuvarı etiketli.

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08:02 min

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August 23, 2018

DOI:

10.3791/58012-v

DOI:
10.3791/58012-v

•

08:02 min
August 23, 2018

•

6 Views
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1Department of Chemistry,Muhlenberg College

Summary

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Burada bir iletişim kuralı için immobilize benchtop metal benzeşme Kromatografi arıtma ve etiketli bir polyhistidine sonraki sulandırma, heme olmayan demir bağlama dioxygenase lisans öğretim laboratuvar için uygun mevcut.

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Colabroy, K. L., Mayer, K. Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory. J. Vis. Exp. (138), e58012, doi:10.3791/58012 (2018).

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