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Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
JoVE Journal
Biochemie
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JoVE Journal Biochemie
Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

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08:02 min

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August 23, 2018

DOI:

10.3791/58012-v

DOI:
10.3791/58012-v

•

08:02 min
August 23, 2018

•

3 Views
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1Department of Chemistry,Muhlenberg College

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Colabroy, K. L., Mayer, K. Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory. J. Vis. Exp. (138), e58012, doi:10.3791/58012 (2018).

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