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Rening och beredning av TRPV1 för spektroskopisk analys

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

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Rening och beredning av TRPV1 för spektroskopisk analys

7,822 Views

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11:53 min

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July 03, 2018

DOI:

10.3791/57796-v

DOI:

10.3791/57796-v

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11:53 min

July 03, 2018

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11 Views

Francisco J. Sierra-Valdez, Richard A. Stein, Phanindra Velissety³, Valeria Vasquez, Julio F. Cordero-Morales

¹Department of Physiology,University of Tennessee Health Science Center, ²Department of Molecular Physiology and Biophysics,Vanderbilt University Medical Center, ³CuriRX, Inc.

Tags

TRPV1 Ion Channel Spectroscopic Analysis Electron Paramagnetic Resonance Double Electron Electron Resonance Conformational Changes Thermal Gating Light-independent Gating PCR Mutagenesis Recombinant Bacmid ETRPV1 Cysteine Mutants

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