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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

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13:13 min

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September 25, 2018

DOI:

10.3791/57776-v

DOI:
10.3791/57776-v

•

13:13 min
September 25, 2018

•

2 Views
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1US Military HIV Research Program, Henry M. Jackson Foundation,Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID,NIH

Summary

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Described is a methodology to quantitate the expression of 96 genes and 18 surface proteins by single cells ex vivo, allowing for the identification of differentially expressed genes and proteins in virus-infected cells relative to uninfected cells. We apply the approach to study SIV-infected CD4+ T cells isolated from rhesus macaques.

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Tokarev, A., Creegan, M., Eller, M. A., Roederer, M., Bolton, D. L. Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques. J. Vis. Exp. (139), e57776, doi:10.3791/57776 (2018).

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