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अलगाव, लक्षण वर्णन, और माउस प्राथमिक गुर्दे ट्यूबलर उपकला कोशिकाओं के उच्च प्रवाह Extracellular फ्लक्स विश्लेषण
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Biología
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JoVE Revista Biología
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
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अलगाव, लक्षण वर्णन, और माउस प्राथमिक गुर्दे ट्यूबलर उपकला कोशिकाओं के उच्च प्रवाह Extracellular फ्लक्स विश्लेषण

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09:40 min

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June 20, 2018

DOI:

10.3791/57718-v

DOI:
10.3791/57718-v

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09:40 min
June 20, 2018

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3 Views
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1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine

Tags

Renal MetabolismFatty Acid OxidationRenal FibrosisNephropathiesMitochondrial BioenergeticsRenal Epithelial CellsHigh-throughput ScreeningRenal Metabolic DiseasePrimary Renal Tubular Epithelial CellsCollagen-coated DishesMouse Kidney PerfusionKidney DigestionTubular Cell Isolation

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Ding, W., Yousefi, K., Shehadeh, L. A. Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells. J. Vis. Exp. (136), e57718, doi:10.3791/57718 (2018).

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