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体外肠道宿主-微生物界面合成细菌联合的可行性评价
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface
DOI:

10:24 min

July 04, 2018

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Capítulos

  • 00:04Título
  • 00:58Development of a Multispecies Community Representative of the Oral Microbiome
  • 02:12Maintenance and Passaging of Caco-2 and HT29-MTX Cell Lines
  • 03:20Assembly of Multicompartment Cell Model Simulating the Gut Host-microbe Interface
  • 05:27Bacterial Survival Following In Vitro Gastrointestinal Transit Conditions
  • 06:50Bacterial Colonization Ability Following In Vitro Gastrointestinal Transit Conditions
  • 08:13Results: Viability of Synthetic Bacterial Community on In Vitro Gut Host Microbe System
  • 09:31Conclusion

Summary

Traducción Automática

采用综合口服群落、体外胃肠消化和小肠上皮模型相结合的新方法对肠道宿主-微生物相互作用进行评价。我们提出了一种方法, 可以适应评估细胞入侵的病原体和多物种生物膜, 甚至测试益生菌制剂的生存性。

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