Journal
/
Neurociencias
/
Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
JoVE Revista
Neurociencias
This content is Free Access.
JoVE Revista Neurociencias
Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

DOI:
10.3791/57648-v

•

08:44 min

•

June 05, 2018

•

,
1College of Nursing,University of Arizona

Capítulos

  • 00:04Título
  • 00:51Skeleton Analysis
  • 05:10Fractal Analysis
  • 07:19Results: Comparison of Data Analysis with and without Application of the Protocol
  • 08:10Conclusion

Summary

Traducción Automática

Traducción Automática

Microglia are brain immune cells that survey and react to altered brain physiology through morphologic changes which may be evaluated quantitatively. This protocol outlines an ImageJ based analysis protocol to represent microglia morphology as continuous data according to metrics such as cell ramification, complexity, and shape.

Tags

Microglia MorphologyImmunohistochemistryImageJSkeleton AnalysisFractal AnalysisNeuroinflammmatoryContinuous VariablesIba1FFT Bandpass FilterUnsharp MaskDespeckleBinarySkeletonize

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Selective Depletion of Microglia from Cerebellar Granule Cell Cultures Using L-leucine Methyl Ester

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Isolation and Culture of Rodent Microglia to Promote a Dynamic Ramified Morphology in Serum-free Medium

Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

Quantifying the Heterogeneous Distribution of a Synaptic Protein in the Mouse Brain Using Immunofluorescence

Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis

Obtaining Human Microglia from Adult Human Brain Tissue

Read Article