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慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法
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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
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慢病毒载体介导的转基因小鼠生产: 一种简单高效的创始人直接研究方法

9,377 Views

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10:21 min

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October 07, 2018

DOI:

10.3791/57609-v

DOI:
10.3791/57609-v

•

10:21 min
October 07, 2018

•

3 Views
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1UPMC Univ Paris 06, INSERM U1127, CNRS UMR 7225, Institut du Cerveau et de la Moelle épinière, ICM,Sorbonne Universités, 2UPMC Univ Paris 06, INSERM UMRS1166, Institute of Cardiometabolism and Nutrition,Sorbonne Universités

Summary

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在这里, 我们提出了一个协议, 以促进转基因基因的整合和生产的创始人转基因小鼠的高效能, 通过简单的注射慢病毒载体载体在 perivitelline 空间的受精卵母细胞。

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Cite this Article

Dussaud, S., Pardanaud-Glavieux, C., Sauty-Colace, C., Ravassard, P. Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders. J. Vis. Exp. (140), e57609, doi:10.3791/57609 (2018).

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