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דור של Huntingtin Exon1 טבעית, לא מתויגות מונומר, הסיבים משתמש באסטרטגיה פיוז'ן סומו
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Capítulos
- 00:04Título
- 00:53Cell Lysis and Purification of His6-SUMO Httex1 Fusion Protein by Immobilized Metal Affinity Chromatography (IMAC)
- 02:56Cleavage of the His6-SUMO-tag and HPLC Purification
- 06:06Disaggregation and Resolubilization of Httex1 Proteins
- 07:53Results: Characterization of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Prepared via SUMO Fusion
- 09:52Conclusion
