Journal
/
Medio ambiente
/
Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
JoVE Revista
Medio ambiente
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Medio ambiente
Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

DOI:
10.3791/57390-v

•

10:14 min

•

May 04, 2018

•

, , ,
1Invasive Insect Biocontrol and Behavior Laboratory, Agricultural Research Service,United States Department of Agriculture, 2Horticultural Research Laboratory, Agricultural Research Service,United States Department of Agriculture

Capítulos

  • 00:05Título
  • 00:57dsRNA Delivery via Green Beans
  • 04:05Foliar Spray Application in Large Potted Citrus Trees and Seedlings
  • 05:06Soil/Root Drench Application in Potted Citrus Trees and Seedlings
  • 05:58Stem Tap Application in Large Potted Citrus Trees and Seedlings
  • 07:06dsRNA Treated Clay Granules for Delivery to Insects Through Soil
  • 08:36Results: Successful Induction of RNAi via dsRNA
  • 09:16Conclusion

Summary

Traducción Automática

Traducción Automática

This article demonstrates novel techniques developed for oral delivery of double-stranded RNA (dsRNA) through the vascular tissues of plants for RNA interference (RNAi) in phloem sap feeding insects.

Tags

Keywords: RNA InterferenceDsRNA DeliveryPhloemPlant-sap-feeding InsectsHemipteran PestsBrown Marmorated Stink BugOral DeliveryGene SilencingMolecular BiopesticidesCapillary ActionGreen Beans4th Instar Nymphs

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

check_url/es/57390?article_type=v

Optimize Flue Gas Settings to Promote Microalgae Growth in Photobioreactors via Computer Simulations
check_url/es/57390?article_type=v

A New Application of the Electrical Penetration Graph (EPG) for Acquiring and Measuring Electrical Signals in Phloem Sieve Elements
check_url/es/57390?article_type=v

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
check_url/es/57390?article_type=v

Methods of Soil Resampling to Monitor Changes in the Chemical Concentrations of Forest Soils
check_url/es/57390?article_type=v

Data Processing Methods for 3D Seismic Imaging of Subsurface Volcanoes: Applications to the Tarim Flood Basalt
check_url/es/57390?article_type=v

Methodology for Developing Life Tables for Sessile Insects in the Field Using the Whitefly, Bemisia tabaci, in Cotton As a Model System
check_url/es/57390?article_type=v

Experimental Methods of Dust Charging and Mobilization on Surfaces with Exposure to Ultraviolet Radiation or Plasmas
check_url/es/57390?article_type=v

Harvesting Venom Toxins from Assassin Bugs and Other Heteropteran Insects
check_url/es/57390?article_type=v

Composition and Distribution Analysis of Bioaerosols Under Different Environmental Conditions
check_url/es/57390?article_type=v

Leaf Area Index Estimation Using Three Distinct Methods in Pure Deciduous Stands

Read Article