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1Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Copenhagen, 2Department of Anesthesiology,Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine,Lund University
Capítulos
- 00:04Título
- 01:17Cannula Preparation
- 01:53Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers
- 05:52Injection of CSF Tracers
- 06:43Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation
- 07:20Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation
- 08:05Results: Analysis of CSF Tracer Distribution
- 09:02Conclusion
Aqui descrevemos um protocolo para realizar a cateterização da cisterna magna (CMc), uma forma minimamente invasiva para entregar traçadores, substratos e moléculas sinalizadoras no fluido cerebrospinal (CSF). Combinado com diferentes modalidades de imagem, CMc permite sistema de glymphatic e avaliação de dinâmica do CSF, bem como a entrega de todo o cérebro de vários compostos.
