Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
1Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Copenhagen, 2Department of Anesthesiology,Yale School of Medicine, 3Center for Translational Neuromedicine, Division of Glial Therapeutics,University of Rochester Medical Center, 4Department of Experimental Medical Science, Wallenberg Center for Molecular Medicine,Lund University
Capítulos
- 00:04Título
- 01:17Cannula Preparation
- 01:53Insertion of the Cannula into the Cisterna Magna for Chronic Injections of CSF Tracers
- 05:52Injection of CSF Tracers
- 06:43Injecting CSF Tracers in Anesthetized Mice with Chronic Cisterna Magna Cannulation
- 07:20Injecting CSF Tracers in Awake Mice with Chronic Cisterna Magna Cannulation
- 08:05Results: Analysis of CSF Tracer Distribution
- 09:02Conclusion
Her beskriver vi en protokoll for å utføre cisterna magna cannulation (CMc), en minimal invasiv måte å levere tracers, underlag og signalnettverk molekyler i spinalvæske (CSF). CMc kombinert med ulike modaliteter for bildebehandling, og kan glymphatic systemet og CSF dynamics vurdering, samt hjernen hele levering av ulike forbindelser.
