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Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
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Capítulos
- 00:00Título
- 00:47Introduction
- 01:33Calibration
- 03:01Sample Preparation
- 05:39Mitochondrial Oxygen Consumption Experiment
- 09:31Representative results
- 13:57Conclusion
In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.
