/
/
Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue
A subscription to JoVE is required to view this content. Sign in or start your free trial.
11,614 Views
•
14:55 min
•
April 07, 2018
DOI:
Summary
Automatically generated
In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.
Read Article
Cite this Article
Simard, C. J., Pelletier, G., Boudreau, L. H., Hebert-Chatelain, E., Pichaud, N. Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue. J. Vis. Exp. (134), e57376, doi:10.3791/57376 (2018).
Copy