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Preparation, Purification, and Use of Fatty Acid-containing Liposomes
JoVE Revista
Bioingeniería
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JoVE Revista Bioingeniería
Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Preparation, Purification, and Use of Fatty Acid-containing Liposomes

25,379 Views

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10:43 min

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February 09, 2018

DOI:

10.3791/57324-v

DOI:
10.3791/57324-v

•

10:43 min
February 09, 2018

•

46 Views
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1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology,Massachusetts General Hospital, 2Department of Biomedical Engineering,Boston University, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Summary

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Liposomes containing single-chain amphiphiles, particularly fatty acids, exhibit distinct properties compared to those containing diacylphospholipids due to the unique chemical properties of single chain amphiphiles. Here we describe techniques for the preparation, purification, and use of liposomes comprised in part or whole of these amphiphiles.

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Jin, L., Engelhart, A. E., Adamala, K. P., Szostak, J. W. Preparation, Purification, and Use of Fatty Acid-containing Liposomes. J. Vis. Exp. (132), e57324, doi:10.3791/57324 (2018).

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