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Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
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Neurociencias
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JoVE Revista Neurociencias
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

DOI:
10.3791/57305-v

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08:35 min

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April 20, 2018

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, , ,
1Department of Neurosurgery,NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

Capítulos

  • 00:05Título
  • 00:42Plate Fabrication
  • 02:02Indenter Block Alignment
  • 04:29Plate Stretching
  • 05:23Membrane Stretch Characterization and Cell Culture Injury
  • 06:58Results: Representative Cell Culture Injury Analyses
  • 08:07Conclusion

Summary

Traducción Automática

Traducción Automática

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.

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Keywords: Human Induced Pluripotent Stem Cell-derived NeuronsHigh-speed Stretch Injury96-well FormatNeurotrauma ModelPhenotypic Drug DiscoveryAPTES CoatingSilicone MembranePlate Fabrication ClampStretching DeviceMotion ControlIn Vitro Neurotrauma

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