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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
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Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
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March 09, 2018
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Here we describe a protocol to determine pulmonary fungal burden in mice with invasive aspergillosis by quantification of Gomori's modified methanamine silver staining in histological sections. Use of this method resulted in comparable results with less animals compared to assessment of fungal burden by quantitative PCR of lung fungal DNA.
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Stolz, D. J., Sands, E. M., Amarsaikhan, N., Tsoggerel, A., Templeton, S. P. Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis. J. Vis. Exp. (133), e57155, doi:10.3791/57155 (2018).
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