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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
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JoVE Revista Bioingeniería
Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

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11:12 min

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August 01, 2018

DOI:

10.3791/57148-v

DOI:
10.3791/57148-v

•

11:12 min
August 01, 2018

•

1 Views
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1Department of Pathology, Faculty of Medicine & Surgery,University of Malta, 2Centre for Molecular Medicine and Biobanking,University of Malta, 3Department of Physiology & Biochemistry, Faculty of Medicine and Surgery,University of Malta

Summary

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Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast cancers.

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RNA-based Expression AssayBreast CancerArchival MaterialLaser MicrodissectionFormalin-fixed Paraffin-embeddedBiomarker ValidationHybridization-based AssayMultiplexSensitivityHeterogeneous Samples

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Cite this Article

Baldacchino, S., Saliba, C., Scerri, J., Scerri, C., Grech, G. Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material. J. Vis. Exp. (138), e57148, doi:10.3791/57148 (2018).

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