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가용성과 불용 성 Huntingtin의 해상도 대 한 분류
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Fractionation for Resolution of Soluble and Insoluble Huntingtin Species
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가용성과 불용 성 Huntingtin의 해상도 대 한 분류

9,287 Views

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07:08 min

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February 27, 2018

DOI:

10.3791/57082-v

DOI:
10.3791/57082-v

•

07:08 min
February 27, 2018

•

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1Department of Psychiatry and Human Behavior,University of California Irvine, 2UCI MIND,University of California Irvine, 3Department of Neurobiology and Behavior,University of California Irvine, 4Board of Governors Regenerative Medicine Institute,Cedars-Sinai Medical Center

Tags

FractionationHuntingtinSolubleInsolubleProtein HomeostasisHuntington’s DiseaseNeurodegenerative DiseaseProtein MisfoldingLysis BufferProtease InhibitorsDounce HomogenizationCell LysateCentrifugationSupernatantPellet

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Cite this Article

Ochaba, J., Morozko, E. L., O’Rourke, J. G., Thompson, L. M. Fractionation for Resolution of Soluble and Insoluble Huntingtin Species. J. Vis. Exp. (132), e57082, doi:10.3791/57082 (2018).

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