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用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测
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An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers
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用荧光标记示踪剂对小鼠血脑屏障通透性的体内检测

23,393 Views

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09:35 min

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February 26, 2018

DOI:

10.3791/57038-v

DOI:
10.3791/57038-v

•

09:35 min
February 26, 2018

•

59 Views
, , ,
1Institute of Neurology (Edinger Institute),Goethe University Hospital, 2Pharmazentrum Frankfurt, Institute for General Pharmacology and Toxicology,Goethe University Hospital

Summary

Automatically generated

在这里, 我们提出了一个小鼠脑血管通透性检测, 采用腹腔注射荧光示踪剂后灌注, 适用于动物模型的血脑屏障功能障碍。一个半脑用于定量评估渗透性, 另一个用于示踪器可视化/染色。10只老鼠的手术过程需要 5-6 小时。

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Cite this Article

Devraj, K., Guérit, S., Macas, J., Reiss, Y. An In Vivo Blood-brain Barrier Permeability Assay in Mice Using Fluorescently Labeled Tracers. J. Vis. Exp. (132), e57038, doi:10.3791/57038 (2018).

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