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Proliferation och differentiering av murina myeloiska föregångare 32D/G-CSF-R celler
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JoVE Revista Biología del desarrollo
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
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Proliferation och differentiering av murina myeloiska föregångare 32D/G-CSF-R celler

9,695 Views

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10:21 min

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February 21, 2018

DOI:

10.3791/57033-v

DOI:
10.3791/57033-v

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10:21 min
February 21, 2018

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2 Views
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1Department of Hemato-Oncology,Institute of Molecular Genetics of the ASCR, 2Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, 2nd Faculty of Medicine,Charles University

Tags

Myeloid Precursor Cells32D-G-CSF-R CellsProliferationDifferentiationGenetic ModificationRetroviral TransductionBosc23 CellsRPMI-1640 MediumInterleukin-3MSCV Retroviral ConstructPCL Echo Packaging VectorPolyethylenimineDMEM MediumFetal Bovine SerumVirus ProductionVirus Titration

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Zjablovskaja, P., Danek, P., Kardosova, M., Alberich-Jorda, M. Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells. J. Vis. Exp. (132), e57033, doi:10.3791/57033 (2018).

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