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アクチンおよび誘導放出の枯渇 (STED) 顕微鏡を用いた B 細胞免疫シナプスにおける微小管細胞骨格の可視化
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Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy
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アクチンおよび誘導放出の枯渇 (STED) 顕微鏡を用いた B 細胞免疫シナプスにおける微小管細胞骨格の可視化

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11:00 min

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April 09, 2018

DOI:

10.3791/57028-v

DOI:
10.3791/57028-v

•

11:00 min
April 09, 2018

•

6 Views
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1Department of Microbiology and Immunology,University of British Columbia

Tags

ActinMicrotubuleCytoskeletonB-cellImmune SynapseSTED MicroscopyCytoskeleton OrganizationCytoskeleton ReorganizationImmune Cell ActivationFluorophoresA20 B-lymphoma CellsTransfectionGoat Anti-mouse Immunoglobulin G AntibodyCoverslips

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Wang, J. C., Bolger-Munro, M., Gold, M. R. Visualizing the Actin and Microtubule Cytoskeletons at the B-cell Immune Synapse Using Stimulated Emission Depletion (STED) Microscopy. J. Vis. Exp. (134), e57028, doi:10.3791/57028 (2018).

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