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Análise clonal de precursores embrionárias células-tronco hematopoiéticas usando índice de célula única classificação combinada com células endoteliais nicho co-cultura
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Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Análise clonal de precursores embrionárias células-tronco hematopoiéticas usando índice de célula única classificação combinada com células endoteliais nicho co-cultura

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09:32 min

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May 08, 2018

DOI:

10.3791/56973-v

DOI:
10.3791/56973-v

•

09:32 min
May 08, 2018

•

3 Views
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1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Department of Pediatrics, Division of Pediatric Hematology/Oncology,University of Washington School of Medicine

Tags

Clonal AnalysisEmbryonic Hematopoietic Stem Cell PrecursorsSingle Cell Index SortingEndothelial Cell Niche Co-cultureDevelopmental HematopoiesisLineage ContributionsHemogenic PrecursorsPhenotypic PropertiesHSC PrecursorsCollagenaseAntibody CocktailFlow CytometrySingle Cell SortingIndex Sorting

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Hadland, B. K., Varnum-Finney, B., Nourigat-Mckay, C., Flowers, D., Bernstein, I. D. Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture. J. Vis. Exp. (135), e56973, doi:10.3791/56973 (2018).

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