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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
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Capítulos
- 00:04Título
- 00:47Cell Growth and Lysis
- 02:23Enrichment of Lipoproteins by TX-114 Phase Partitioning
- 05:05SDS-PAGE, Electroblotting, and Staining with Ponceau S
- 06:30Tryptic Digestion, Lipopeptide Extraction from Nitrocellulose Membrane, Deposition onto MALDI Target, and Data Acquisition
- 08:47Results: MALDI-TOF MS Analysis of N-terminal Lipopeptides
- 10:17Conclusion
The enrichment of bacterial lipoproteins using a non-ionic surfactant phase partitioning method is described for direct use in TLR assays or other applications. Further steps are detailed to prepare N-terminal tryptic lipopeptides for structural characterization by mass spectrometry.
