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Immunology and Infection

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JoVE Journal Immunology and Infection
Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
 

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Article DOI: 10.3791/56842-v • 10:59 min • May 21st, 2018
May 21st, 2018 •
1, 1
1Department of Biochemistry and Molecular Biology, Pennsylvania State University

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The enrichment of bacterial lipoproteins using a non-ionic surfactant phase partitioning method is described for direct use in TLR assays or other applications. Further steps are detailed to prepare N-terminal tryptic lipopeptides for structural characterization by mass spectrometry.

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Keywords: Bacterial Lipoproteins N-terminal Lipopeptides Structural Determination Mass Spectrometry MALDI-TOF Toll-like Receptor Immune Response TX-114 Surfactant Phase Separation Cell Disruption Zirconia Silica Beads Centrifugation
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