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Visualisation de SNARE intracellulaire traite par Fluorescence Lifetime Imaging Microscopy
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
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Visualisation de SNARE intracellulaire traite par Fluorescence Lifetime Imaging Microscopy

9,154 Views

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08:55 min

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December 29, 2017

DOI:

10.3791/56745-v

DOI:
10.3791/56745-v

•

08:55 min
December 29, 2017

•

7 Views
, , ,
1Department of Tumor Immunology,Radboud University Medical Center

Summary

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Ce protocole décrit une nouvelle méthode permettant la visualisation quantitative de la formation d’un complexe de protéines SNARE, basée sur le transfert d’énergie Förster et durée de vie de fluorescence imaging microscopy.

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Cite this Article

Verboogen, D. R. J., Baranov, M. V., ter Beest, M., van den Bogaart, G. Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy. J. Vis. Exp. (130), e56745, doi:10.3791/56745 (2017).

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