/

Inmunología y contagio

/

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

JoVE Revista
Inmunología y contagio

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Revista Inmunología y contagio

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Please note that all translations are automatically generated. Click here for the English version.

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

DOI:

10.3791/56689-v

•

10:35 min

•

December 26, 2017

•

Lisa Kozicky, Laura M. Sly

¹British Columbia Children’s Hospital Research Institute,University of British Columbia

Capítulos

00:05Título
00:44Bone Marrow Macrophage Derivation and Activation with Antibodies: In Vitro Testing
05:34Harvesting Peritoneal Macrophages after an IVIg + LPS Challenge: In Vitro Testing
08:38Results: BMDM Reaction to IVIg + LPS Treatment
10:06Conclusion

Summary

Traducción Automática

抗体药物对炎症性疾病进行了革命性的治疗。除了对特定靶点产生直接影响外, 抗体还能激活巨噬细胞成为抗炎药。该协议描述了如何抗炎巨噬细胞活化可以评估在体外,使用小鼠骨髓巨噬细胞, 和在体内,使用腹腔巨噬细胞。

Tags

Antibody-based Drugs Murine Bone Marrow Peritoneal Macrophages Anti-inflammatory IL-10 Immunology Primary Macrophages Bone Marrow In Vitro Peritoneal Cavity In Vivo Euthanized Mouse Bone Marrow Isolation Bone Marrow Culture MCSF

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

激活的B细胞CD40的小鼠模型

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

使用骨髓源性巨噬细胞巨噬细胞极化的调查

骨髓衍生的巨噬细胞产

隔离和小鼠骨髓的静脉注射单核细胞衍生

跟踪小鼠骨髓单核细胞<em>在体内</em

鼠后肢长骨解剖和骨髓隔离

股骨窗口商会模型<em>在体内</em在小鼠骨髓>手机跟踪

分离及小鼠淋巴细胞的激活

粒子结合Bet v 1的变应原PM10的流式细胞分析

Read Article