Journal
/
Neurociencias
/
产后大鼠听觉脑干反应及外毛细胞全细胞贴片钳记录
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
Please note that all translations are automatically generated. Click here for the English version.

产后大鼠听觉脑干反应及外毛细胞全细胞贴片钳记录

DOI:
10.3791/56678-v

•

09:23 min

•

May 24, 2018

•

, , , , ,
1Department of Physiology, School of Basic Medical Sciences,Southern Medical University, 2School of Basic Medical Sciences,Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region,Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences,Southern Medical University

Capítulos

  • 00:04Título
  • 00:56Auditory Brainstem Response Recording
  • 02:12The Organ of Corti Dissection
  • 03:22Immunofluorescence Staining
  • 05:21Patch Clamp Recording of Isolated OHCs
  • 07:33Results: Whole-Cell Patch Clamp Recording of Isolated Outer Hair Cells
  • 08:19Conclusion

Summary

Traducción Automática

Traducción Automática

本协议描述的方法, 记录的听觉脑干反应的产后鼠幼崽。为研究外发细胞的功能发育情况, 本文分步介绍了全细胞膜片钳在分离外毛细胞中的实验过程。

Tags

Auditory Brainstem ResponseOuter Hair CellWhole-cell Patch ClampPostnatal RatsCochlear Hair CellsAuditory FunctionElectrophysiologyInner EarCochleaOrgan Of CortiLeibovitz’s L-15 MediumDissection

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

膜片钳记录在内耳毛细胞的分离斑马鱼

全细胞膜片钳红外神经刺激机制的调查

从Morphologically-和确定的Neurochemically海马的interneurons全细胞膜片钳记录

在脑片全细胞膜片钳记录

全细胞贴片记录用于电泳生理测定通道视紫质中的离子选择性

爪蟾Tectal 神经元全细胞膜片钳记录的制备与协议

c57bl6 小鼠耳蜗植入术与电唤起脑干反应记录

凝胶神经元全细胞斑块夹的急性脊髓切片的制备

全细胞膜片电生理方法对突触倍数的评价

鸡幼崽听觉脑干反应的评价

Read Article