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拟南芥线粒体的分离和呼吸测量

JoVE Revista
Bioquímica

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JoVE Revista Bioquímica

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

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拟南芥线粒体的分离和呼吸测量

DOI:

10.3791/56627-v

•

09:54 min

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January 05, 2018

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Wenhui Lyu, Jennifer Selinski, Lu Li, David A. Day, Monika W. Murcha, James Whelan, Yan Wang

¹ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, ²School of Biological Sciences,Flinders University, ³ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Capítulos

00:05Título
00:50Preparation of Grinding Medium, Wash Buffer, and Gradient Solutions
02:22Homogenization and Mitochondrial Isolation
06:30Oxygen Consumption Measuurements
08:42Results: Isolation of Purified Mitochondrial Protein and Oxygen Consumption Measurement of Purified Mitochondria Obtained from Arabidopsis thaliana Plants
09:37Conclusion

Summary

Traducción Automática

由于线粒体只占植物细胞的一小部分, 它们需要被纯化以进行一系列的研究。线粒体可以通过同质化的各种植物器官分离, 其次是微分和密度梯度离心, 以获得高纯度的线粒体分数。

Tags

Keywords: Mitochondria Isolation Arabidopsis Thaliana Respiration Mitochondrial Composition Biogenesis Metabolism Energy Biology Stress Tolerance Gradient Centrifugation PVP Gradient Grinding Filtration

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