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En effektiv og enkel metode til at etablere NK og T-cellelinier fra patienter med kronisk aktiv Epstein - Barr virusinfektion
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1Genetic Resources Center, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences, 2MOE Key Laboratory of Major Diseases in Children, National Key Discipline of Pediatrics, National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, 3State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology,Chinese Academy of Sciences
Capítulos
- 00:04Título
- 01:35Isolation of PBMC from CAEBV Patients
- 02:59Expansion of Cells and Numbering the Viable Cells
- 04:08Preparation for Cell Phenotyping by Flow Cytometry
- 05:52Expansion and Cryopreservation of NK/T Cells
- 07:23Results: Representative Gating Strategy for Flow Cytometry Analysis of Cell Lines L196 and M296
- 08:25Conclusion
