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Inmunología y contagio

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血液循环中稀有抗原特异 B 细胞的鉴别人单克隆抗体的生成

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

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血液循环中稀有抗原特异 B 细胞的鉴别人单克隆抗体的生成

10,149 Views

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13:14 min

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February 06, 2018

DOI:

10.3791/56508-v

DOI:

10.3791/56508-v

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13:14 min

February 06, 2018

•

5 Views

Marie-Claire Devilder^1,2,3, Mélinda Moyon^1,2,3, Xavier Saulquin^1,2, Laetitia Gautreau-Rolland^1,2

¹CRCINA, Inserm, CNRS, Université d’Angers,Université de Nantes, ²LabEx IGO, “Immunotherapy, Graft, Oncology”, ³CHU Nantes, France

Summary

我们描述了一种生产人类抗体特异性抗原的方法, 从人类血液中的稀有 B 细胞开始。这些天然抗体的产生是高效和快速的, 而获得的抗体可以区分高度相关的抗原。

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