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Strukturelle Charakterisierung von Mannan Zellwand Polysaccharide in Anlagen, in denen Tempo
JoVE Revista
Bioquímica
This content is Free Access.
JoVE Revista Bioquímica
Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
DOI:

11:06 min

October 16, 2017

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Capítulos

  • 00:05Título
  • 00:37Aliquoting and Pre-treatment of Alcohol Insoluble Residue (AIR)
  • 02:11Hydrolysis of AIR with Glycosyl Hydrolases
  • 03:20Derivitization of Oligosaccharides
  • 04:29Preparation of PACE Gels
  • 06:33Running a PACE Gel
  • 07:51Visualizing a PACE Gel
  • 09:07Results: Characterization of Plant Cell Wall Glucomannam Structure
  • 10:30Conclusion

Summary

Traducción Automática

Ein Protokoll für die Strukturanalyse von Polysacchariden durch Gelelektrophorese (PACE), mit Mannan als Beispiel, wird beschrieben.

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