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激光诱导的斑马鱼视网膜变性及再生模型中的 m ü ller 胶质细胞活化

JoVE Revista
Neurociencias

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JoVE Revista Neurociencias

Müller Glia Cell Activation in a Laser-induced Retinal Degeneration and Regeneration Model in Zebrafish

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激光诱导的斑马鱼视网膜变性及再生模型中的 m ü ller 胶质细胞活化

9,444 Views

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06:27 min

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October 27, 2017

DOI:

10.3791/56249-v

DOI:

10.3791/56249-v

•

06:27 min

October 27, 2017

•

7 Views

Federica M. Conedera^1,2,3, Petra Arendt¹, Carolyn Trepp^1,2,3, Markus Tschopp¹, Volker Enzmann^1,2

¹Department of Ophthalmology, University Hospital of Bern,University of Bern, ²Department of Clinical Research,University of Bern, ³Graduate School for Cellular and Biomedical Sciences,University of Bern

Summary

斑马鱼是研究脊椎动物视网膜变性/再生机制的流行动物模型。本议定书描述一种方法, 以诱导局部损伤破坏外视网膜, 对内视网膜的损害最小。随后, 我们监测在体内视网膜形态学和 m ü ller 胶质细胞反应在整个视网膜再生。

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