Journal
/
Neurociencias
/
Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy
JoVE Revista
Neurociencias
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Neurociencias
Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

DOI:
10.3791/56237-v

•

08:38 min

•

October 23, 2017

•

,
1Division of Anatomy, Institute of Basic Medical Sciences,University of Oslo, 2Department of Microbiology,Oslo University Hospital

Capítulos

  • 00:05Título
  • 00:46Viral Transduction
  • 01:52Microscope Set-up
  • 03:24Time-lapse Imaging
  • 06:52Results: Time-lapse Imaging to Track Mitochondrial Movement in Oligodendrocytes
  • 08:12Conclusion

Summary

Traducción Automática

Traducción Automática

Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.

Tags

OligodendrocyteOrganelleOrganotypic Brain SliceAdeno-associated VirusConfocal MicroscopyMitochondriaMyelinTime-lapse ImagingViral Transduction

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice

Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation

Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Read Article