/
/
Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:12Título
- 00:48Construction of photobleaching aparatus and solutions
- 02:30Photobleaching pre-treatment of tissue
- 03:37Immunofluorescence
- 09:35Fluorescence microscopy
- 12:13Representative results
- 15:58Conclusion
Background autofluorescence of biological samples often complicates fluorescence-based imaging techniques, especially in aged human postmitotic tissues. This protocol describes how autofluorescence from these samples can be effectively removed using a commercially available light emitting diode light source to photobleach the sample prior to immunostaining.
