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窒素キャビテーションと差動遠心分離培養細胞の周辺膜蛋白質の分布を監視することができます。
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Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells
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窒素キャビテーションと差動遠心分離培養細胞の周辺膜蛋白質の分布を監視することができます。

15,392 Views

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08:24 min

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August 18, 2017

DOI:

10.3791/56037-v

DOI:
10.3791/56037-v

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08:24 min
August 18, 2017

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13 Views
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1Langone Medical Center,New York University

Tags

Nitrogen CavitationDifferential CentrifugationPeripheral Membrane ProteinsCultured CellsSubcellular CompartmentalizationHEK293 CellsHomogenization BufferPressurizationProtease InhibitorCell DisruptionFractionationSoluble FractionMembrane Fraction

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Zhou, M., Philips, M. R. Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells. J. Vis. Exp. (126), e56037, doi:10.3791/56037 (2017).

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