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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
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A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

8,721 Views

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10:04 min

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July 06, 2017

DOI:

10.3791/56013-v

DOI:
10.3791/56013-v

•

10:04 min
July 06, 2017

•

1 Views
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1Department of Biochemistry and Molecular Biology,University of Texas Health Science Center at Houston

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Cross-linked Skeletal MuscleChromatin ImmunoprecipitationNuclei IsolationFiltration-based MethodMuscle Genomic StudiesTime-sensitive ContextFormaldehyde Cross-linkingDounce HomogenizerProtease Inhibitors

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Nohara, K., Chen, Z., Yoo, S. A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation. J. Vis. Exp. (125), e56013, doi:10.3791/56013 (2017).

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